Large Study Identified New Genetic Link to Male Infertility (Biology)

The findings published in eLife show that men with this unstable subtype of the Y chromosome have a significantly increased risk of genomic rearrangements. These rearrangements affect the sperm production process (spermatogenesis) and consequently, these men can be up to nine times more likely to have fertility issues. Molecular diagnostics of this genetic variant could help identify those at higher risk in their early adulthood, giving them the chance to make decisions about future family planning early on. Currently, the exact cause of infertility remains unknown in more than half of men with spermatogenic impairment.

In the large-scale study led by geneticist Pille Hallast and conducted in cooperation between the Human Genetics Research Group of the University of Tartu Institute of Biomedicine and Translational Medicine, the Andrology Centre of Tartu University Hospital and the Wellcome Sanger Institute, the Y chromosomes of more than 2,300 Estonian men were analysed. The men were involved in the study at the Andrology Centre of Tartu University Hospital by the team of Professor Margus Punab.

The Y-chromosomal region studied in this paper has been associated with male infertility before, but this is the first time the genetic variation of such a large clinical sample has been investigated. According to Hallast, this is the most sophisticated in-depth analysis of the genetic variation of Y chromosome in patients with spermatogenic impairment.

One of the senior co-authors of the study, Professor of Human Genetics of the University of Tartu Maris Laan said that the previously undescribed subtype of Y chromosome discovered in cooperation with colleagues from the UK causes a high risk of severe spermatogenic impairment. “At one point during evolution, the ‘ancestor’ of these Y chromosomes experienced the inversion of a long DNA segment, causing instability in this Y chromosome and predisposing to the deletion of surrounding DNA segments,” explained Laan.

This inverted Y chromosome variant is relatively common and does not always lead to partial deletion of DNA or fertility issues. Therefore, it can be passed down in families and remain unnoticed until an offspring has infertility issues. According to estimates, in this Y lineage, the deletion of genetic material that harms spermatogenesis occurs in about one man in ten or even less often. This subtype is carried by a significant number of men of European descent, but it is not found as widely in other continents. In Estonia, it can be found in 5-6% of men, but in Poland and Czechia, the figure is nearly 20%.

The world’s leading population geneticist Dr. Chris Tyler-Smith, collaborator working at the Wellcome Sanger Institute, underlined the need for more synergetic research between teams in population and reproductive genetics to map unfavourable Y-chromosomal variants linked to impaired spermatogenesis and understand how these have evolved and survived demographically.

Professor Maris Laan emphasised the potential practical value of molecular diagnostics of this research, as early identification of the genetic reason of male spermatogenic impairment could allow a more focused clinical management of infertile couples. “While the deletions in the studied Y chromosome region were previously known to interfere with sperm production, we now know that the deletion of DNA sequences leads to the genetic predisposition to infertility depending on the Y-chromosomal lineage,” she added.

Featured image: The prevalence of the Y-chromosomal haplogroup R1a1-M458 carrying a fixed r2/r3 inversion. (A) Geographical distribution of haplogroup R1a1-M458 and its sub-lineages in Europe. Pie charts indicate populations, with the black sector showing the proportion of R1a1-M458 according to Underhill et al., 2015. (B) The estimated proportion of subjects among idiopathic cases with severe spermatogenic failure (sperm counts 0-10 × 106/ejaculate) carrying R1a1-M458 Y lineage (and its sub-lineages) chromosomes that have undergone a subsequent partial AZFc deletion. The prevalence was estimated using reported population frequencies of R1a1-M458, including Estonians (Underhill et al., 2015) and data available in the current study for Estonian men with spermatogenic failure. Estonians are shown in bold and with a striped filling. © University of Tartu


Reference: Pille Hallast et al., “A common 1.6 mb Y-chromosomal inversion predisposes to subsequent deletions and severe spermatogenic failure in humans”,elife, 2021. https://elifesciences.org/articles/65420


Provided by Estonian Research Council

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