HKUMed Develops Inhaled Dry Powder Formulation of Broad-spectrum Antiviral Against COVID-19 and Influenza (Medicine)

The collaborative research team formed by the Department of Pharmacology and Pharmacy and Department of Microbiology, LKS Faculty of Medicine, The University of Hong Kong (HKUMed) has developed an inhalable dry powder formulation of tamibarotene, a repurposed drug that exhibits broad-spectrum antiviral activity against SARS-CoV-2, MERS-CoV and influenza A H1N1 virus following pulmonary delivery. The work has been published online in Advanced Therapeutics [link to publication] in June 2021. A provisional application has been filed to the United States Patent and Trademark Office based on this work.

Background

In response to the unpredictable epidemic and pandemic outbreaks of influenza and coronavirus, early administration of a broad-spectrum antiviral agent could be an effective strategy to control the spread of respiratory infections. The HKUMed research team has previously reported that a retinoid derivative, AM580, demonstrated broad-spectrum antiviral activity against coronaviruses (MERS-CoV, SARS-CoV and SARS-CoV-2) and influenza virus (Nature Communication 2019Viruses 2020).

An analogue of AM580, tamibarotene, is an orally active retinoid derivative for the treatment of acute promyelocytic leukemia currently marketed in Japan. With an antiviral activity comparable to AM580, tamibarotene has an established safety profile in human with milder adverse effects than other retinoid derivatives. Tamibarotene is available as an oral tablet, which is difficult to achieve robust antiviral activity in the respiratory tract due to inadequate lung distribution following oral administration. When high dose is administered to compensate the insufficient lung distribution, toxic side effects are expected because of the extensive systemic exposure.

Photos of ‘spray-freezing’ step in spray freeze drying process. Liquid is fed into a nozzle and atomised into fine droplets. Those droplets fall into liquid nitrogen (inside the white styrofoam container) and become frozen. © University of Hong Kong

By contrast, pulmonary delivery is a non-invasive administration route that can maximise local concentration in the lung and reduce systemic exposure, thereby reducing the risk of adverse effects and improving therapeutic efficacy. Inhaled powder formulation of broad-spectrum antiviral drug can be used on outpatient basis due to the ease of (self-)administration as prophylaxis and treatment for respiratory viral infections.

Study method and findings

Inhaled dry powder formulation of tamibarotene was prepared by spray freeze drying, a particle engineering technique that combines spray freezing and freeze drying to produce particles with excellent aerosol properties for inhalation. The HKUMed research team found that a prophylactic dose of tamibarotene powder delivered by intratracheal administration significantly reduced virus titer and viral RNA load of SARS-CoV-2 in the hamsters’ lungs, and the antiviral efficacy was comparable to intratracheally administered remdesivir. The broad-spectrum anti-coronavirus activity of inhaled tamibarotene powder was also demonstrated in MERS-CoV-infected mice model as pre-challenge prophylaxis. Moreover, remarkable anti-influenza activity of tamibarotene powder formulation was demonstrated by the improved survival rate of mice and alleviated disease severity when administered either intratracheally as prophylaxis or intranasally as treatment.

Research implications

‘Currently, there is no inhaled powder formulation of antiviral available on the market for COVID-19 treatment. Tamibarotene dry powder with broad-spectrum antiviral activity presents a new strategy for COVID-19 management, especially as prophylaxis and treatment for outpatients when in-patient healthcare cannot be provided,’ said Dr Jenny Lam, Associate Professor, Department of Pharmacology and Pharmacy, HKUMed, who initiated the study. ‘With the favourable safety profile of tamibarotene and based on the findings in the present study, clinical trials evaluating inhaled tamibarotene for its safety and as at-home treatment for COVID-19 could be considered.’

Since tamibarotene exerts its antiviral activity by targeting lipid metabolism in the host cells, it is advantageous in responding to mutated or drug-resistant virus strains. More importantly, by demonstrating antiviral efficacy against SARS-CoV-2 and H1N1 virus in respective animal models, inhaled tamibarotene presents a possible solution for COVID-19 and influenza co-infection.

Featured image: SEM image of ‘inhaled tamibarotene powder formulation’ at x5,000 magnification. © University of Hong Kong


Provided by University of Hong Kong

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s