Researchers ID Location on Brain Protein Linked to Parkinson’s Disease Development (Neuroscience)

Johns Hopkins Medicine researchers say they have pinpointed the section of alpha-synuclein, a protein in the brain, that causes it to latch onto brain cells called neurons and likely drives the development of Parkinson’s disease, a progressively worsening disorder that disrupts movement and neurological functions. The findings may help scientists develop a treatment that curbs the protein’s improper binding, perhaps slowing or stopping the progression of Parkinson’s.

Results of the study, conducted using cell studies and mouse neurons, were published online June 25, 2021, in the Proceedings of the National Academy of Science.

In Parkinson’s disease, the alpha-synuclein protein can misfold and take an abnormal shape, enabling it to bind and clump onto a neuron’s surface. Clumps of misfolded alpha-synuclein, known as alpha-synuclein fibrils, spread to other healthy neurons and ultimately kill these cells as the fibrils pile up.

“These findings are significant because we determined what part of alpha-synuclein fibrils is important in the binding process and how it leads to Parkinson’s progression,” says study co-author Xiaobo Mao, Ph.D., assistant professor of neurology at the Institute for Cell Engineering of the Johns Hopkins University School of Medicine.

Through several molecular and cell studies, the research team focused on a region at the tip of the misfolded alpha-synuclein fibrils called the C terminus, along with the addition of p129, a chemical group near the end of the fibril. The researchers knew from previous studies that both of these characteristics enable the fibrils to bind to neurons and cause cell death.

Mao and his Johns Hopkins Medicine colleagues — including study co-author and Parkinson’s disease researcher Ted Dawson, M.D., Ph.D., who is director of the Institute for Cell Engineering and professor of neurology at the Johns Hopkins University School of Medicine — determined the C terminus can bind receptors on the neurons due to the receptors’ positive electrical charges. When the receptors were removed, the C terminus could not clump to neurons and spread to other healthy cells.

In healthy alpha-synuclein, the C terminus is covered and cannot bind to neurons.

The researchers also investigated p129, a group of molecules in alpha-synuclein fibrils that have an extra chemical group, and are present in about 95% of people with Parkinson’s and other Lewy body neurodegenerative diseases, such as Lewy body dementia. When the alpha-synuclein fibrils with p129 and the C terminus were injected into mouse neurons, researchers found that those fibrils bound with neuron receptors and spread faster. This contributed to quicker cell death and disease progression.

To slow the progression of clumping alpha-synuclein fibrils, the researchers hope to develop treatments that target the binding process.

“If the spreading of alpha-synuclein fibrils can be blocked by preventing the C terminus from binding, it would be a good therapeutic strategy,” says Mao.

Featured image: Researchers at Johns Hopkins Medicine have pinpointed the section of a brain protein called alpha-synuclein that enables it to latch onto neurons and likely drives the development of Parkinson’s disease, a progressively worsening neurological disorder. The photomicrograph shows an aggregation of alpha synuclein in brain tissue taken from a patient with Parkinson’s disease. Credit: Public domain image courtesy of Suraj Rajan


Reference: Shengnan Zhang et al, Mechanistic basis for receptor-mediated pathological α-synuclein fibril cell-to-cell transmission in Parkinson’s disease, Proceedings of the National Academy of Sciences (2021). DOI: 10.1073/pnas.2011196118


Provided by Johns Hopkins University School of Medicine

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