As part of a trans-Atlantic collaboration, researchers from the team of Kodi Ravichandran (VIB-UGent Center for Inflammation Research) and the University of Virginia School of Medicine (Charlottesville, Virginia, USA) have discovered how intestinal bacteria can exploit nutrients that are released from dying cells as fuel to establish intestinal infections.
One cell’s death…
Researchers have long been investigating two seemingly distinct fields of study: how certain bacteria colonize our intestinal tracts, and how our own cells die. But do those processes interact?
Kodi Ravichandran (VIB-UGent Center for Inflammation Research), says: “We have known for a few decades that the cell death process itself can indirectly influence bacterial infections by changing the body’s immune response. At the same time, we have also been studying how dying cells can communicate with their neighbors. What CJ (CJ Anderson, postdoctoral fellow) set out to ask was: if these dying cells are secreting factors that can be recognized and sensed by healthy neighboring cells, what is to stop other organisms like intestinal bacteria from recognizing these same molecules?”
Using cell culture and healthy mouse tissue systems, the authors discovered that certain molecules are actively produced and shed by intestinal epithelial cells when they start to die. Interestingly, these molecules are directly sensed and used by intestinal bacteria such as Salmonella and E. coli.
…is the other one’s DINNR
The researchers showed that this relationship between dying cells and gut bacteria, which they term death-induced nutrient release (DINNR), takes place in several disease contexts. Intestinal bacteria can use cell death-related molecules to assist in colonization during food poisoning, inflammatory conditions (resembling Inflammatory Bowel Disease (IBD) or Crohn’s Disease), and chemotherapy-induced mucositis. Gastrointestinal toxicity is one of the leading side effects in cancer patients undergoing chemotherapy, and it can necessitate dose alterations that can reduce efficacy. Additionally, cancer patients receiving chemotherapy have a significantly greater risk of developing subsequent infections.
“This relationship between chemotherapy and bacterial infections was particularly interesting to us,” says Anderson. “Unlike foodborne infections or flareups of IBD or Crohn’s Disease where a patient does not know when he or she will be ‘under attack’, physicians know exactly when they are administering chemotherapeutic drugs to cancer patients. This means we have a therapeutic window where we can try to develop some sort of combination therapy to limit some of this fuel for bacteria.”
The researchers also revealed that restricting the release of certain nutrients from dying cells, without having to stop the cell death process itself, was able to protect animals from infection. According to Anderson, this could have major implications for developing future therapeutics. “What was exciting to us was that we do not need to cheat death itself to still see some protection. We do not need to go searching for the Holy Grail. If we can change or restrict some of these nutrients that are released during death, we can try to improve patient care. As with any piece of fundamental research, there is more work to be done, but the opportunity is there for future therapies.”
Featured image credit: VIB (the Flanders Institute for Biotechnology)
Reference: Christopher J. Anderson et al, Microbes exploit death-induced nutrient release by gut epithelial cells, Nature (2021). DOI: 10.1038/s41586-021-03785-9
Provided by VIB